STAMP FOR CELL DISSOCIATION, METHOD FOR CELL DISSOCIATION USING THE SAME, AND APPARATUS FOR MANUAL/AUTOMATICAL CELL DISSOCIATION USING THE SAME

摘要

A stamp for cell dissociation, a method for cell dissociation by using the same stamp, and an apparatus for manual/automatic cell dissociation by using the same stamp are provided to dissociate the human and animal cells into the cell units having a certain cell number at one time and divide them into a certain pattern according to a purpose. The stamp(100) for dissociation of human and animal cells comprises a body(110) having an upper side and a lower side, and a micro-pattern portion(120) which is located at any one of the upper and lower sides, and has at least one repeated micro-pattern(130) with a certain height and width, wherein the micro-pattern portion is made of polydimethylsiloxane(PDMS), polymethylmethacrylate(PMMA), polyacrylates, polycarbonates, polycyclic olefins, polyimides, polyurethanes or polyester. The method for cell dissociation comprises the steps of: (a) preparing cell population in a culture dish; (b) placing the stamp on the culture dish in parallel; (c) pressing the culture dish with the stamp for a certain time to dissociate the cell population; and (d) removing the stamp.