发明名称 |
Cell-based assays employing voltage and calcium dyes |
摘要 |
Disclosed are novel compositions and methods employing cyclic nucleotide-gated channels and fluorescence dyes and other indicators in detecting changes of intracellular cAMP levels, for instance in response to the stimulation of G-protein-coupled receptors. CNG mutants comprising one or more pore mutations that enhance sensitivity to cAMP and decrease divalent cation-mediated blockage are used in a variety of assays, including detection of the G-protein-coupled receptor activity. Also disclosed are novel dye quencher formulations that are particularly suited for cell-based assays of protein interactions.
|
申请公布号 |
US7238213(B2) |
申请公布日期 |
2007.07.03 |
申请号 |
US20040978282 |
申请日期 |
2004.11.01 |
申请人 |
ATTO BIOSCIENCE |
发明人 |
YAO YONG;CAO LIANG;LU JIANMING;LLORENTE ISABEL |
分类号 |
C09B51/00;A61B5/0275;C07H21/04;C07K14/705;C12N15/09;G01N33/567 |
主分类号 |
C09B51/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|