| 摘要 |
Metallic ion independent isomerase and a method for isomerization of sugars by using the same enzyme are provided to prevent loss of isomerization activity in the enzyme when metallic ion is released by EDTA(ethylenediamine tetraacetic acid) treatment, and induce non-specific conversion of aldose-ketose. The metallic ion independent isomerase has the amino acid sequences of SEQ ID NO:7 and SEQ ID NO:8 and is encoded by genes having the nucleotide sequences of SEQ ID NO:5 and SEQ ID NO:6. The metallic ion dependent isomerase is produced by transforming a host cell such as Escherichia coli with an expression vector containing the genes of metallic ion independent isomerase, and culturing the transformed host cell. The ketose selected from tagatose, ribulose, fructose, xylulose and psicose is produced from aldose by using the metallic ion independent isomerase. The aldose selected from D-glucose, D-galactose, D-xylose, D-mannose, D-allose, D-fucose, D-ribose, L-arabinose, D-arabinose and L-ribose is produced from ketose by using the metallic ion independent isomerase.
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