METHOD OF PURIFYING THROMBIN-LIKE PROTEASE ENZYMES OBTAINED FROM SNAKE VENOM

摘要

A PROCESS FOR PURIFYING THROMBIN-LIKE PROTEASES FROM SNAKE VENOMS IS DESCRIBED,WHICH CONSISTS IN FREEING, THE PROTEASES FROM IMPURITIES IN THREE CHROMATOGRAPHIC STEPS: A) AFFINITY OR ANION EXCHANGE, B) ADSORPTION ONTO A GLASS MATRIX AT ALKALINE PH VALUES, AND C) SIZE EXCLUSION GEL OR GLASS MATRIX AT ACIDIC PH VALUES.