Methods and systems for acquiring real-time quantitative melt data

摘要

Described are methods and systems for acquiring real-time quantified melt data. A first Double-stranded nucleic acid are immobilized on a support. The temperature of the double-stranded nucleic acids is slowly ramped up until the double-stranded nucleic acids melt. Differences in fluorescence emission are used to signal when the melt occurred. An evanescent field is used to generate fluorescence emission.