发明名称 |
Species-specific probes for identification of target virus and identification method using said probes |
摘要 |
The inventions provides a method for identifying a target virus in an infected subject comprising the steps of designing a pair of degenerate primers corresponding to highly conserved regions of the target virus; designing a pair of species-specific primers according to highly variable sequences within the conserved regions of the target virus; preparing the species-specific probes according to the larger sequence variations within the conserved regions of the target virus, which are amplified with the species-specific primers as obtained; preparing a test sample by amplifying total nucleic acid of the infected subject with the degenerate primers as obtained; contacting the test sample with the species-specific probes as obtained; and detecting a hybridization between the species-specific probe and the test sample, wherein the hybridization indicates the target virus is identified in the infected subject. The primers and probes for detecting a garget virus are also provided.
|
申请公布号 |
US2007148637(A1) |
申请公布日期 |
2007.06.28 |
申请号 |
US20050318907 |
申请日期 |
2005.12.27 |
申请人 |
BUREAU OF ANIMAL AND PLANT HEALTH INSPECTION AND QUARANTINE, COUNCIL OF AGRICULTURE, EXECUTIVE YUAN |
发明人 |
CHANG YA-CHUN;HSU YUEH-CHWEN;YEH TZE-JUNG |
分类号 |
C12Q1/70;C07H21/04;C12Q1/68 |
主分类号 |
C12Q1/70 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|