METHOD FOR PROPAGATING ADENOVIRAL VECTORS ENCODING INHIBITORY GENE PRODUCTS
摘要
The invention provides a method of propagating an adenoviral vector. The method comprises (a) providing a cell comprising a cellular genome comprising a nucleic acid sequence encoding a tetracycline operon repressor protein (tetR), and (h) contacting the cell with an adenoviral vector comprising a heterologous nucleic acid sequence encoding a toxic protein. The heterologous nucleic acid sequence is operably linked to a promoter and one or more tetracycline operon operator sequences (tetO), and expression of the heterologous nucleic acid sequence is inhibited in the presence of tetR, such that the adenoviral vector is propagated. The invention also provides a system comprising the aforementioned cell and adenoviral vector.
申请公布号
WO2007073513(A2)
申请公布日期
2007.06.28
申请号
WO2006US60732
申请日期
2006.11.08
申请人
GENVEC, INC.;GALL, JASON, G., D.;BROUGH, DOUGLAS, E.;RICHTER, KING, C.
发明人
GALL, JASON, G., D.;BROUGH, DOUGLAS, E.;RICHTER, KING, C.