Method and device for determining the ligand concentration in a sample

摘要

Determining the concentration of ligands contained in a sample, comprises contacting the sample at test place, detecting four measured values representing the frequency of the bonding between the ligand and the receptors (5) present on the test place and determining the concentration of the ligands in the sample by the four measured values. A saturation solution contains the ligands in higher concentration than the sample. All the receptors are bonded at the ligands and are immobilized on a carrier. Determination of the concentration of ligands contained in a sample, comprises contacting the sample at test place, detecting four measured values representing the frequency of the bonding between the ligand and the receptors (5) present on the test place and determining the concentration of the ligands in the sample by the four measured values. A saturation solution contains the ligands in higher concentration than the sample. All the receptors are bonded at the ligands and are immobilized on a carrier. The sample exhibits two different types of ligands. Each ligand-type is assigned to each receptor-type respectively. A first test place receptor of a first receptor-type and a second test place receptor of a second receptor type are immobilized on the carrier. Luminophores and/or chemiluminophores are bounded directly and/or indirectly at the ligands by detection of antibody as a function of the bonding of the ligands at the receptors. The ligands induce the luminophores and/or chemiluminophores for the discharge of luminescence radiation, and the first and second measured values are detected through measurement of the luminescence radiation. On the carrier, two test places are arranged, on which same and different receptors are immobilized with more different density. An independent claim is included for a device for the determination of the concentration of ligands contained in a sample.