SEPARATION METHOD FOR HERBICIDAL EXTRACTS FROM THE EPIMEDIUM KOREANUM NAKAI

摘要

An extract of Epimedium koreanum Nakai comprising fractions of EADBA, RADBB and EADBC is provided to show high herbicidal activity, thereby being applicable as a natural herbicidal agent. The method comprises the steps of: (a) extracting Epimedium koreanum Nakai with an organic solvent such as methanol, ethanol, propanol, butanol, and pentanol and then drying it; (b) dissolving the product obtained from the step(a) in ethylacetate to obtain an ethylacetate fraction; (c) subjecting the ethylacetate fraction to absorption column chromatography using a mixture solution of ethylacetate, dichloromethane, and methanol so as to obtain an EA fraction having the herbicidal activity; (d) subjecting the EA fraction to absorption column chromatography using a mixture solution of ethylacetate, dichloromethane, and methanol so as to obtain a EAD fraction having the herbicidal activity; (e) subjecting the EAD fraction to absorption column chromatography using a mixture solution of methanol, acetonitrile, and water to obtain a EADB fraction having the herbicidal activity; and (f) subjecting the EADB fraction to absorption column chromatography using a mixture solution of methanol and water so as to obtain a EADBA fraction, a EADBB fraction or a EADBC fraction having the herbicidal activity. The herbicidal composition comprises the extract obtained from the Epimedium koreanum Nakai.