发明名称 |
SEPARATION OF PHOTOSENSITIZER ISOMERS AND STEREOISOMERS BY LASER-INDUCED FLUORESCENCE CAPILLARY ELECTROPHORESIS |
摘要 |
A method for the separation of isomers and stereoisomers of photosensitizers by Laser-Induced Fluorescence Capillary Electrophoresis has been developed. The limits of detection are 2.06 x 10-6 M, and the relative standard deviation for the separation was 2.90 % to 4.64 %. Benzoporphyrin derivative mono and diacid (BPD-MA, BPD-DA) enantiomers ca n be quantitatively determined in the range of 10-2 to 10-5 mg mL-1. In comparison with HPLC, CE has better resolution and efficiency. This separation method was successfully applied to the BPD enantiomers obtained from a matrix of bovine serum and from liposomally formulated material as well as from studies with rat, dog and human microsomes.
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申请公布号 |
CA2353827(C) |
申请公布日期 |
2007.06.26 |
申请号 |
CA19992353827 |
申请日期 |
1999.12.10 |
申请人 |
UNIVERSITY OF BRITISH COLUMBIA |
发明人 |
PENG, XUEJUN;DOLPHIN, DAVID;STERNBERG, ETHAN D. |
分类号 |
B01D57/02;G01N27/447 |
主分类号 |
B01D57/02 |
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