发明名称 |
Method for identifying a biosynthetic pathway gene product |
摘要 |
The present invention provides isolated, genetically modified host cells, where a host cell is genetically modified with a nucleic acid that includes a nucleotide sequence encoding a biosynthetic pathway enzyme. Synthesis of the enzyme in the host cell results in conversion of a substrate for the enzyme into a biosynthetic pathway intermediate, which intermediate is produced in an amount effective to inhibit growth of the genetically modified host cell. The present invention further provides compositions and kits comprising a subject genetically modified host cell. Subject host cells are useful for identifying a gene product having activity in a biosynthetic pathway. The present invention further provides methods of identifying a gene product having activity in a biosynthetic pathway.
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申请公布号 |
US2007141574(A1) |
申请公布日期 |
2007.06.21 |
申请号 |
US20040573796 |
申请日期 |
2004.09.29 |
申请人 |
KEASLING JAY D;NEWMAN JACK D;PITERA DOUGLAS J;WITHERS SYDNOR T III;REILING KEITH K;MARTIN VINCENT J |
发明人 |
KEASLING JAY D.;NEWMAN JACK D.;PITERA DOUGLAS J.;WITHERS SYDNOR T.III;REILING KEITH K.;MARTIN VINCENT J. |
分类号 |
C12Q1/68;C07H21/04;C12N;C12N15/00;C12N15/09;C12N15/74 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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