METHOD AND SYSTEM FOR PHASE-LOCKED SEQUENCING

摘要

System and methods according to exemplary embodiments of the present disclosure utilize a sample holder configured to hold at least one confined single-molecule analyte in a solution of labeled nucleotide bases. Each single- molecule analyte has a single template nucleic acid molecule, an oligonucleotide primer, and/or a single nucleic acid polymerizing enzyme. A least one light source is used to illuminate a detection volume around each confined analyte, and a pulsed source sends a pulsed radiation to the at least one detection volume. The timing of incorporation events at the analytes are controlled by the pulsed radiation, and when multiple analytes are provided on the sample holder, the incorporation events at the analytes can be phase locked and synchronized using the pulsed radiation.