METHODS FOR CULTURING MESENCHYMAL STEM CELL AND COMPOSITIONS COMPRISING MESENCHYMAL STEM CELL FOR REPARING SKIN DEFECTS

摘要

The present invention relates to a method for culturing a mesenchymal stem cell highly expressing collagen, which comprises the steps of: (a) culturing a mesenchymal stem cell isolated from an adipose tissue in a serum-containing medium; and (b) culturing the mesenchymal stem cell in a serum-free medium, wherein the expression level of type I pro-collagen of the mesenchymal stem cell cultured in the step (b) is higher than that of fibroblast and the expression level of pro-MMP-1 (pro-matrix metalloprotease-1) of the mesenchymal stem cell cultured in the step (b) is lower than that of fibroblast. In addition, the present invention relates to a pharmaceutical composition for repairing a skin defect comprising mesenchymal stem cells as active ingredients.