发明名称 |
HEPATITIS C VIRUS NS2/3 ASSAY |
摘要 |
The present invention provides an assay for the detection of the NS2/3 cleavage products NS2 or NS3 in the presence of uncleaved NS2/3. Following self-cleavage of NS2/3 to generate NS2 and NS3 cleavage products, a sample is incubated with a ligand specific for the recognition of NS2 or NS3 cleavage product in the presence of uncleaved NS2/3. There is provided a method for detecting a NS2/3 autocleavage product in a sample containing NS2/3 protease, whereby the amount of bound ligand detected correlates with the NS2/3 autocleavage activity. A further aspect of the present invention concerns ligands selectively recognizing one of the NS2 cleavage product or the NS3 cleavage product with minimal cross-reactivity with the uncleaved NS2/3 and the other cleaved product. The present invention provides antibodies that selectively recognize one of cleaved NS2 product or cleaved NS3 product with minimal cross-reactivity with the uncleaved NS2/3 and the other cleaved product.
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申请公布号 |
US2007141701(A1) |
申请公布日期 |
2007.06.21 |
申请号 |
US20050275284 |
申请日期 |
2005.12.21 |
申请人 |
BOEHRINGER INGELHEIM INTERNATIONAL GMBH |
发明人 |
LAMARRE LYNE;LAGACE LISETTE;THIBEAULT DIANE |
分类号 |
C12Q1/68;C12N5/06;C12N5/16 |
主分类号 |
C12Q1/68 |
代理机构 |
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地址 |
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