发明名称 |
MODIFIED SMALL INTERFERING RNA MOLECULES AND METHODS OF USE |
摘要 |
<p>The present invention provides double-stranded RNA molecules that mediate RNA interference in target cells, preferably hepatic cells. The invention also provides double-stranded RNA (dsRNA) molecules that are modified to be resistant to nuclease degradation, which inactivates a virus, and more specifically, hepatitis C virus (HCV). The invention also provides a method of using these modified RNA molecules to inactivate virus in mammalian cells and a method of making modified small interfering RNAs (siRNAs) using human Dicer. The invention provides modified RNA molecules that are modified to include a dsRNA or siRNA wherein one or more of the pyrimidines in the RNA molecule are modified to include 2′-Fluorine. The invention also provides dsRNA or siRNA in which all pyrimidines are modified to include a 2′-Fluorine. The invention provides that the 2′-Fluorine dsRNA or siRNA molecule is further modified to include a two base deoxynucleotide “TT” sequence at the 3′ end of the molecule.</p> |
申请公布号 |
EP1796732(A2) |
申请公布日期 |
2007.06.20 |
申请号 |
EP20050808592 |
申请日期 |
2005.09.30 |
申请人 |
NOVARTIS VACCINES AND DIAGNOSTICS, INC. |
发明人 |
HAN, JANG;HOUGHTON, MICHAEL |
分类号 |
C12N15/113;A61K31/22;A61K31/7105;A61K48/00;C12Q1/70 |
主分类号 |
C12N15/113 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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