| 摘要 |
A mixing vessel for containing a liquid comprises a chamber having a lower chamber portion, an upper chamber portion wider than the lower portion, a gas inlet means 14 for supplying gas to the lower portion and means for redirecting rising gas 24. In use the rising gas, in the form of bubbles, initially rises substantially vertically and is redirected in a substantially horizontal direction by means for redirecting rising gas. The mixing vessel is particularly used for cell culture. The gas inlet comprises a porous ceramic tube extending across a rear wall of the vessel and the vessel further includes additional ports 36,37 for the addition of liquid reagents and/or gases. A temperature regulation plate 42 is provided at the back wall and a sensor 44 is provided on the front wall which can be used to monitor operating parameter. An elevated pressure of about 0-2 PSI can be maintained in the headspace 29 by the action of a pressure regulation valve 38 with an associated filter assembly 40. Headspace pressure, pH and oxygen saturation can be controlled via a control unit. |
