NOVEL ENDOCHITINASE AND SANGUIBACTER SP. HAVING THE ABILITY PRODUCING THE SAME

摘要

A novel endochitinase and Sanguibacter sp. producing the same endochitinase are provided to show high activity of the endochitinase at various temperature including cool temperature and pH ranges, and stable activity in the presence of surfactants, so that the novel endochitinase and its producing microorganism are useful for production of chitinoligomer from chitin. A gene encoding the novel endochitinase has the nucleotide sequence of SEQ ID NO:1. The Sanguibacter sp. KCTC 10714BP produces the novel endochitinase encoded by the gene of SEQ ID NO:1. The novel endochitinase is produced by (a) culturing Sanguibacter sp. KCTC 10714BP or transformed Escherichia coli containing the endochitinase gene in a medium containing 0.1-1% of chitin as a carbon source at 4-25 deg.C, and (b) recovering the novel endochitinase from the cultured medium.