A PREPARTION METHOD FOR A PROTEIN WITH NEW FUNCTION THROUGH SIMULTANEOUS INCORPORATION OF FUNCTIONAL ELEMENTS

摘要

Disclosed is a method for preparing a protein having a new function. The method comprises (A) a functional element-designing step of designing functional elements required for a new function desired to impart to an existing protein scaffold; (B) a functional element- inserting step of simultaneously inserting at least two gene fragments corresponding to the designed functional elements into a protein scaffold gene; and (C) a mutant screening and improving step of screening a mutant having a new function from a library of mutants inserted with the mutant genes, and improving and optimizing the function of the screened mutant using a directed evolution technique. The method for preparing can be widely used for the development of therapeutic proteins and the creation of industrial enzymes in the fields of bioengineering and biotechnology.