Dengue virus detection measured by immunocytometry in a dendritic cell surrogate

摘要

The invention concerns a method for diagnosing dengue invention early after dengue virus infection. The method comprises binding dengue virus contained in sera from infected patients with dentritic cell-specific intracellular adhesion molecule (ICAM) 3-grabbing nonintegrin (DC-SIGN) or liver/lymph node-specific intracellular adhesion molecule (ICAM) 3-grabbing nonintegrin (L-SIGN). DC-SIGN or L-SIGN can be expressed in transfected B cells or adhered to other surfaces. Measurement of binding is by immunocytometry, microscopy or other antibody based methods.