MARKED BOVINE VIRAL DIARRHEA VIRUS VACCINES

摘要

The present invention is directed to a bovine viral diarrhea virus comprising
at least one helicase domain amino acid mutation wherein the mutation in the
NS3 domain results in a loss of recognition by a monoclonal antibody raised
against wild-type NS3 but wherein viral RNA replication and the generation of
infectious virus is retained. The present invention is useful, for example, to
produce a marked bovine viral diarrhea virus vaccine or to differentiate
between vaccinated and infected or unvaccinated animals.