摘要 |
A conditionally live virion and method for making the same whereby the viral DNA or RNA is modified so that the virion is incapable of replication unless a protein supplement is added to the expression system. The expression system is either a traditional cell culture or cell free expression system suitable fo r self assembly of viral particles. Both expression systems require the additi on of viral proteins either for replication or assembly of the replication incompetent virion. The conditionally live viron is then used for creating a vaccine with three fold . immunogenic properties that are elicited by 1) the whole intact replication incompetent virus; 2) the conditionally live virion temporally resuscitated by addition of protein supplements; and 3) the prote in supplement itself acting as a subunit vaccine.
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