METHOD FOR PREPARATION OF cRNA

摘要

Disclosed is a method for preparation of cRNA, which is characterized in that the reduction in the yield of cRNA can be prevented. The method comprises the steps of: (a) performing a reaction for treating a mRNA-cDNA hybrid prepared by reverse transcription reaction with RNase H to prepare single-stranded cDNA and a reaction for preparing double-stranded cDNA from the single-stranded cDNA, and subsequently deactivating the RNase contained in the reaction solution; (b) contacting the reaction solution with a solid substrate having a cationic group on its surface under such pH conditions that the cationic group can be charged positively; (c) separating the solid substrate from the reaction solution; (d) eluting the double-stranded cDNA from the solid support; and (e) performing a transcription reaction for preparing cRNA from the double-stranded cDNA.