Stable cyanine dye phosphoramidites

摘要

This invention provides dye phosphoramidites, particularly phosphoramidites of substituted cyclic bridged cyanine and related dyes, of the general formula: In this formula, each dotted line represents carbon atoms necessary to form a fused substituted or unsubstituted aromatic ring; m is an integer from 1 to 18; Y and Z are independently selected from the group consisting of S, O, N, CH₂ and C(CH₃)₂; R₁ is an alkyl; (PAM) is a phosphoramidite group; X^{{circle around (-)}} is a negative ion; and Q is L-W, wherein L is a conjugated cyclic moiety and W is OR₂, wherein R₂ is a second alkyl. Methods of making and using the dye phosphoramidites are also provided.