| 摘要 |
<p>The present invention relates generally to a nucleic acid expression system and methods for use therein. More particularly, the present invention provides a bicistronic expression system which, due to the design of a novel selection mechanism, exhibits improved selection efficiency relative to known bicistronic expression systems. The construct of the invention comprises an IRES operably linked to a selection marker. The expression system of the present invention now facilitates the high level expression of both soluble and membrane bound forms of a protein of interest. The transfected host cell systems, and molecule produced thereby, which utilise the nucleic acid expression system of the present invention are useful, inter alia, in a wide variety of diagnostic, therapeutic, prophylatic and research based settings.</p> |
