发明名称 |
Two-color (two-photon) excitation with focused excitation beams and a raman shifter |
摘要 |
Two-color (two-photon) excitation with two confocal excitation beams is demonstrated with a Raman shifter as excitation light source. Two-color excitation fluorescence is obtained from Coumarin 6H dye sample (peak absorption=394 nm, peak fluorescence=490 nm) that is excited using the first two Stokes outputs (683 nm, 954 nm, two-color excitation=398 nm) of a Raman shifter pumped by a 6.5 nsec pulsed 532 nm-Nd:YAG laser (Repetition rate=10 Hz). The two Stokes pulses overlap for a few nanoseconds and two-color fluorescence is generateven with focusing objectives of low numerical apertures (NA<=0.4). We observed the linear dependence of the two-color fluorescence signal with the product of the average intensities of the two Stokes excitation beams. The two-color fluorescence distribution is strongly localized around the common focus of the confocal excitation beams.
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申请公布号 |
US2007128733(A1) |
申请公布日期 |
2007.06.07 |
申请号 |
US20020529043 |
申请日期 |
2002.09.27 |
申请人 |
SALOMA CAESAR A;PALERO JONATHAN A;GARCIA WILSON O |
发明人 |
SALOMA CAESAR A.;PALERO JONATHAN A.;GARCIA WILSON O. |
分类号 |
G01N21/62;G01N21/63;G02B21/00 |
主分类号 |
G01N21/62 |
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