发明名称 |
SOLID PHASE RFLP-BASED SNP DETECTION |
摘要 |
<p>The invention is directed to a method for detection of single nucleotide polymorphisms utilizing a solid phase RFLP-based method of detection. The invention further relates to a method of detecting a single nucleotide polymorphism (SNP) comprising providing a sample test DNA molecule containing an SNP site of interest; detectably labeling the DNA molecule at or near each of its ends with different labels and containing the SNP site of interest; providing at least two different immobilization oligonucleotides capable of immobilization to a solid support; digesting the test DNA molecule with a restriction endonuclease, whose cut site contains the SNP site in at least one allele of the SNP site; separating strands of the test DNA molecule; annealing the strands to the immobilization oligonucleotides; and detecting the labels of DNA strands annealed to the immobilization oligonucleotide.</p> |
申请公布号 |
WO2007065028(A2) |
申请公布日期 |
2007.06.07 |
申请号 |
WO2006US46354 |
申请日期 |
2006.12.04 |
申请人 |
GENE CHECK, INC. |
发明人 |
SCHELL, ALLAN, C.P.;DANIELL, ERICA, L. |
分类号 |
C12Q1/68;C07H21/04;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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