Novel carbonyl reductase, gene encoding it and process for producing optically active alcohols using the same

摘要

A polypeptide comprises: (1) an amino acid sequence (i) with 251 amino acids; or (2) an amino acid sequence based on the sequence (i) but with some amino acids deleted, added or substituted and having carbonyl reductase activity. Independent claims are also included for: (1) a DNA containing: (a) a base sequence encoding a polypeptide with an amino acid sequence (i); (b) a base sequence encoding a variant of the polypeptide; (c) a base sequence (ii) with 753 base pairs; (d) a base sequence derived from the sequence (ii) but with some bases deleted, added or substituted and encoding a polypeptide with carbonyl reductase activity; and (e) a base sequence hybridizable with the base sequence (ii), its complementary sequence or a part of them under stringent conditions and encoding a polypeptide with carbonyl reductase activity; (2) a recombinant DNA obtained by integrating the DNA into a vector; (3) transformant sustaining the recombinant DNA, or obtained by integrating the DNA into a chromosomal DNA; and (4) a process for producing a compound of formula (IV) by culturing a carbonyl compound of formula (I), (II) or (III) with the transformant cells, their culture or processed material to action on the carbonyl compound for asymmetric reduction. [Image] R : H, alkyl or aryl.