Hydrophilic marker on the basis of diastereomeric cyanines

摘要

Hydrophilic marker based on cyanine diastereomer (I) and/or (II) is new. Hydrophilic marker based on cyanine diastereomer of formula (I) and/or (II) is new. R 1, R 2aliphatic (preferably methyl) or heteroaliphatic group; L, L 1-L 4divalent linear -(CH 2) o-, branched or cyclic alkylene (optionally interrupted by one or more O, substituted N and/or S atom); o : 1-15; X 1-OH, -SH, NH 2, -NH-NH 2, -F, -Cl, -Br, -I, -O-succinimidyl, O-sulfosuccinimidyl, -O-(4-sulfo-2,3,5,6-tetrafluorophenoxy), -O-(2,3,5,6-tetrafluorophenoxy), benzotriazole, -NR-L-OH, -NR-L-O-phosphoramidite, -NR-L-SH, -NR-L-CO-NH 2, -NR-L-CO-NH-NH 2, -NR-L-CHO, -NR-L-COOH, -NR-L-COO-succinimidyl, NR-L-COO-sulfosuccinimidyl, -NR-L-CO-O-4-sulfo-2,3,5,6-tetrafluorophenoxy, -NR-L-CO-O-2,3,5,6-tetrafluorophenoxy, -NR-L-NH-CO-CH 2-I or -NR-L-maleimide; R : H, R 1or R 2; X 2, X 3H, alkyl, tert.alkyl, aryl, carboxyaryl, dicarboxyaryl, heteroaryl, cycloalkyl, heterocycloalkyl, alkoxy, alkylmercapto, aryloxy, arylmercapto, OH, amino, NO 2, CN or solubilizing and/or ionizable and/or ionized group e.g. SO 3->, PO 32->, CO 2->, tert-ammonium, cyclodextrin or sugar; Y 1halo (F, Cl or Br), substituted phenoxy or substituted arylmercapto (phenylsulfanyl); Cation : Na +>, K +>, Ca 2+>, ammonium or other cation to balance negative charge in (I) and/or (II); n : 0-3; R 3, R 4aliphatic (preferably methyl) or heteroaliphatic group; or R 3+R 4-(CH 2) m-, -(CH 2) mO(CH 2) m-, -(CH 2) mS(CH 2) m-, -(CH 2) mCH=CH- or -OCH=CH-; and m : 2-6. [Image] [Image].