Process for the enzymatic synthesis of triglycerides

摘要

Enzyme-catalyzed synthesis of esters of polyunsaturated fatty acid, short-chained and/or sensitive fatty acids with higher triglyceride, comprises synthesis of fatty acids or their alkyl esters with glycerin into its triglyceride in presence of an enzyme under vacuum or arid conditions; reverse hydrolysis of the obtained triglyceride by adding water in the presence of an enzyme under normal pressure; and separation of the fatty acids and/or alkyl esters and glycerin by distillation or refining of triglycerides. Independent claims are included for: (1) a fatty acid glyceride ester, obtained by the above process; (2) fatty acid ester of poly unsaturated fatty acid with triglyceride of at least 90%; (3) fatty acid ester of conjugated linolic acid with triglyceride of at least 90%; (4) fatty acid ester of medium chain fatty acid with triglyceride of at least 90%; and (5) fatty acid ester comprising a mixture of medium chain fatty acid and/or conjugated linolic acid and/or poly unsaturated fatty acid with a triglyceride of at least 90%.