发明名称 |
METHOD FOR ENRICHMENT AND/OR ISOLATION OF DOPAMINERGIC NEURONS |
摘要 |
A method which comprises transferring reporter nucleic acid molecules, which express a fluorescent protein under the regulation by the promoter/enhancer of a gene expressed in dopaminergic neurons, into individual cells of a cell ma ss and separating fluorescing cells from this cell mass. A method which compris es transferring the above-described reporter nucleic acid molecules into individual cells of a cell mass and measuring the fluorescence distribution of the cell mass to thereby visualize and identify the dopaminergic neurons occurring in the cell mass in a viable state. A method of identifying a dopaminergic neuron inductive factor which comprises transferring a reporter nucleic acid molecule into cells capable of differentiating into dopaminergi c neurons, allowing these cells to coexist with a candidate substance and then determining whether or not the candidate substance is a dopaminergic neuron inductive factor with the use of the fluorescence of the cells as an indication.
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申请公布号 |
CA2381065(C) |
申请公布日期 |
2007.06.05 |
申请号 |
CA20002381065 |
申请日期 |
2000.12.07 |
申请人 |
JAPAN SCIENCE AND TECHNOLOGY CORPORATION |
发明人 |
KOBAYASHI, KAZUTO;SAWAMOTO, KAZUNOBU;MATSUSHITA, NATSUKI;OKANO, HIDEYUKI |
分类号 |
C12N5/10;C12N5/0793;C12N15/09;C12Q1/02;C12Q1/04;C12Q1/68 |
主分类号 |
C12N5/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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