Product e.g. make-up product, color strength evaluation method for e.g. make-up field, involves comparing coloration of reconstructed epidermis with trial epidermis not in contact with product to be evaluated

摘要

#CMT# #/CMT# The method involves applying a product to be evaluated on a stratum corneum of a reconstructed epidermis comprising keratocyte. The product is in contact with the reconstructed epidermis for a time period between 5 minutes to 48 hours with a relative humidity between 50 and 90 percentages. The coloration of the reconstructed epidermis is compared with a trial epidermis not in contact with the product to be evaluated. #CMT# : #/CMT# An independent claim is also included for a reconstructed epidermis utilized in a color strength evaluating method #CMT#USE : #/CMT# Used for evaluating color strength of a product such as colored or make-up products e.g. pigment or red/blue colorant, on skin (claimed) and for evaluating covering effect of formulation of pigmentary mole e.g. ageing mole, or skin coloring e.g. tattooing, in a field of self-tan or make-up. #CMT#ADVANTAGE : #/CMT# The method permits to effectively reproduce characteristics of ethnic skin, already colored skin or pigmentary mole and to obtain reliable and reproducible coloration. #CMT#INORGANIC CHEMISTRY : #/CMT# The pre-coloration is obtained by divalent or trivalent iron salt. The coloration is obtained by catalyst system comprising salt, zinc oxide or manganese oxide. #CMT#ORGANIC CHEMISTRY : #/CMT# Preferred Material: The reconstructed epidermis is subjected to pre-coloration that is obtained by compound selected among dihydroxyacetone, erythrulose, cysteine or glutathione. The coloration is obtained by 5, 6-dihydroxyindole-2-carboxylic acid or 5,6-dihydroxyindole, cysteine or catalyst system comprising alkaline hydrogen carbonate.