摘要 |
<P>PROBLEM TO BE SOLVED: To provide a specific and quantitative amplification method for gene, when the expression of the gene is measured. <P>SOLUTION: By using mRNA as a template cDNA is synthesized, then the cRNA is amplified, and only desired gene is reverse transcribed with a gene-specific primer. Further, the cRNA included in the specimen is decomposed to obtain the specimen including only the single-stranded DNA originating from the desired gene. <P>COPYRIGHT: (C)2007,JPO&INPIT |