| 摘要 |
<p>A method of detecting Shigella apyrase enzyme comprises the steps of : a) contacting a sample suspected of containing said Shigella apyrase enzyme withan endo-pyrophosphatase substrate comprising an endo pyrophosphate group that is susceptible to cleavage by said enzyme, said cleavage yielding a signalogenspecies comprising a monophosphate group, said signalogen species being susceptible to phosphatase-induced formation of a signalophore species; b) providing a phosphatase activity so as to convert said signalogen species into a signalophore species; c) monitoring for a signal from said signalophore species as an indication of the presence of said Shigella apyrase enzyme. The essay can be used for the detection of Shigella spp. or enteroinvasive E. CoIi.</p> |
