DELAYED EXPRESSION VECTORS

摘要

The invention, in certain embodiments, relates to nucleic acid sequences comprising (a) a transcription unit encoding a chimeric protein composed of a composite DNA-binding domain and a transcription activation domain, (b) a transcription unit encoding an immune stimulator expressed under the control of a minimal promoter and an enhancer comprising one or more DNA binding site(s) for the composite DNA-binding domain, and (c) a transcription unit encoding an immunogen, wherein the composite DNA-binding domain comprises a continuous polypeptide chain containing two or more component polypeptide domains, at least two of which are mutually heterologous, and related expression constructs, methods of achieving delayed expression of immune stimulators, methods of inducing an immune response, and methods of immunization.