发明名称 Malic acid production in recombinant yeast
摘要 We disclose a recombinant yeast, wherein the yeast is pyruvate decarboxylase enzyme (PDC) activity negative (PDC-negative) and is functionally transformed with a coding region encoding a pyruvate carboxylase enzyme (PYC) wherein the PYC is active in the cytosol, a coding region encoding a malate dehydrogenase enzyme (MDH) wherein the MDH is active in the cytosol and is not inactivated in the presence of glucose, and a coding region encoding a malic acid transporter protein (MAE). We also disclose a method of producing malic acid by culturing such a yeast in a medium comprising a carbon source and a carbon dioxide source and isolating malic acid from the medium.
申请公布号 AU2006317058(A1) 申请公布日期 2007.05.31
申请号 AU20060317058 申请日期 2006.11.01
申请人 TATE & LYLE INGREDIENTS AMERICAS, INC. 发明人 AARON ADRIAAN WINKLER;JACOBUS THOMAS PRONK;ABRAHAM FREDERIK DE HULSTER;JOHANNES PIETER VAN DIJKEN
分类号 C12N1/00;C12N1/16;C12P7/46 主分类号 C12N1/00
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