METHODS FOR PRODUCTION OF RECOMBINANT ALPHA1-ANTITRYPSIN

摘要

Methods of producing properly folded recombinant al -antitrypsin (AAT) polypeptide are provided. Denatured recombinant AAT polypeptide is refolded by first solubilizing the polypeptide with a chaotroph at high pH, followed by refolding in the presence of reduced concentrations of chaotroph and in the presence of PEG, glycerol or sucrose, or a detergent while the pH is slowly reduced and is generally maintained.