发明名称 |
DNA DECONTAMINATION METHOD |
摘要 |
The invention is related to a method for amplifying a methylated target nucleic acid in a sample while avoiding amplification of a non-methylated target nucleic acid by inactivating it. This is accomplished by a restriction enzyme digest after bisulfite treatment of the target nucleic acid. The invention is further related to the use of a restriction enzyme to avoid amplification of a non-methylated target nucleic acid while amplifying a methylated target nucleic acid in a sample and kits for performing the methods according to the invention. |
申请公布号 |
EP1789586(A2) |
申请公布日期 |
2007.05.30 |
申请号 |
EP20050784458 |
申请日期 |
2005.09.02 |
申请人 |
ROCHE DIAGNOSTICS GMBH;F.HOFFMANN-LA ROCHE AG |
发明人 |
BERGMANN, FRANK;MARKERT-HAHN, CHRISTINE |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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