| 摘要 |
The present invention provides a method of modifying chromosomes, using chromosome splitting vectors. In a preferred embodiment, a method is provided for modifying yeast chromosomes, using linear chromosome splitting vectors. <??>The method of the invention includes preparing a first linear chromosome splitting vector comprising a first target sequence, a marker gene sequence, and a first (C4A2)n sequence; preparing a second linear chromosome splitting vector comprising a second target sequence, a centromere sequence of a chromosome, and a second (C4A2)n sequence; and introducing the chromosome splitting vectors into a cell, wherein n is independently an integer of 1 to 30, preferably 4-15, more preferably 6-10. <IMAGE> |
