发明名称 |
METHOD OF DETECTING SPECIFIC FRAGMENTS OF DNA OR RNA WITH THE AID OF A REAL-TIME POLYMERASE CHAIN REACTION |
摘要 |
Methods of amplifying specific fragments of DNA or RNA with the aid of a polymerase chain reaction in real time are disclosed. The methods include providing a first oligonucleotide primer that comprises a donor fluorescent dye and a second oligonucleotide primer that comprises an acceptor fluorescent dye; allowing the primers to anneal to a target nucleic acid at positions abutting to each other; carrying out a polymerase chain reaction which allows for a fluorescent resonant transfer of energy between the donor dye and the acceptor dye; detecting an increase of fluorescent emission of the acceptor dye; and correlating the increase in the emission of the acceptor dye with the accumulation of the specific fragment of DNA or RNA. In one embodiment, the primers comprise a fluorescent dye and a universal quencher.
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申请公布号 |
US2007117125(A1) |
申请公布日期 |
2007.05.24 |
申请号 |
US20060553223 |
申请日期 |
2006.10.26 |
申请人 |
INSTITUT BIOKHIMII I GENETIKI UFIMSKOGO NAUCHNOGOTSENTRA RAN |
发明人 |
CHEMERIS ALEXEI V.;NIKONOROV YURY M.;ROMANENKOVA MAIYA L.;CHEMERIS DMITRY A.;GARAFUTDINOV RAVIL R.;MAGAZOVA ROZA A.;MALEEV GRIGORY V.;VAKHITOV VENER A.;VASILOV RAIF G. |
分类号 |
C12Q1/68;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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