发明名称 |
WHOLE BLOOD SAMPLE PREPARATION FOR POLYMERASE CHAIN REACTIONUSING AMMONIUM CHLORIDE AND A CARBOXYLIC ACID OR METAL CARBOXYLATE FOR SELECTIVE RED BLOOD CELL LYSIS |
摘要 |
Leucocytes can be quickly and selectively separated from erythrocytes by subjecting a whole blood sample to a series of steps including lysing the erythrocytes and washing the remaining leucocytes with a solution containing ammonium chloride and a carboxylic acid or a metal carboxylate. The resulting white blood cells can be readily lysed and subjected to polymerase chain reaction to amplify and detect a target nucleic acid. The test kit useful in practicing the amplification method includes a labeled primer, a PCR reagent and a reagent mixture containing ammonium chloride and a carboxylic acid or a metal carboxylate for sample preparation.
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申请公布号 |
CA2199337(C) |
申请公布日期 |
2007.05.22 |
申请号 |
CA19972199337 |
申请日期 |
1997.03.06 |
申请人 |
JOHNSON & JOHNSON CLINICAL DIAGNOSTICS, INC. |
发明人 |
EKEZE, TOBIAS E.;KERSCHNER, JOANNE HANSEN |
分类号 |
C12Q1/68;G01N33/48;C12N1/06;C12N5/06;C12N5/08;C12N15/09;C12N15/10;C12P19/34;C12Q1/70;G01N33/49;G01N33/50 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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