摘要 |
<p>Method for the identification of a gene in a transposon population, comprising isolating genomic DNA, optionally pooling the DNA, restrict the DNA in the pools using an enzyme, ligate adaptors, amplifying the adaptor-ligated fragments with primers, one of which is a primer complementary to a border of a transposon sequence, high throughput sequencing of the fragments, aligning the fragments with known sequences in a database and thereby identifying gene candidates.</p> |
申请人 |
KEYGENE N.V.;VAN EIJK, MICHAEL, JOSEPHUS, THERESIA;GERATS, ANTONIUS, GERARDUS, MARIE;VAN TUNEN, ADRIANUS, JOHANNES;VANDENBUSSCHE, MICHIEL, MARCEL, ALBERT |
发明人 |
VAN EIJK, MICHAEL, JOSEPHUS, THERESIA;GERATS, ANTONIUS, GERARDUS, MARIE;VAN TUNEN, ADRIANUS, JOHANNES;VANDENBUSSCHE, MICHIEL, MARCEL, ALBERT |