发明名称 |
EXPRESSION SYSTEM INCORPORATING A CAPSID PROMOTOR SEQUENCE AS AN ENHANCER OF A CYTOMEGALOVIRUS PROMOTOR |
摘要 |
The invention provides a method for enhancing expression of a transgene in a host cell, which includes the steps of inserting a capsid promoter element (Pcap), or a sequence comprising the reverse complement of the capsid promoter element (PcapR), into a mammalian expression cassette upstream (5') of a cytomegalovirus immediate/early enhancer/ promoter region (Pcmv); inserting the transgene into the expression cassette downstream (3') of the cytomegalovirus immediate/early enhancer/ promoter region; inserting a vector containing the expression cassette into the host organism; and causing expression of the transgene. The capsid promoter element (Pcap) is typically from a circovirus, parvovirus or anellovirus. The transgene is typically expressed at a higher level than when expressed by a vector containing the expression cassette without the transcriptional control element. An expression cassette, vector, DNA vaccine, pharmaceutical composition and method of treatment are also claimed. |
申请公布号 |
WO2007054788(A1) |
申请公布日期 |
2007.05.18 |
申请号 |
WO2006IB03150 |
申请日期 |
2006.11.08 |
申请人 |
SOUTH AFRICAN MEDICAL RESEARCH COUNCIL;UNIVERSITY OF CAPE TOWN;RYBICKI, EDWARD PETER;TANZER, FIONA LESLEY |
发明人 |
RYBICKI, EDWARD PETER;TANZER, FIONA LESLEY |
分类号 |
C12N15/85;A61K39/21 |
主分类号 |
C12N15/85 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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