| 摘要 |
[Problems] To provide a method which comprises detecting phosphorylation of substrate peptides with a quick and easy scheme using an inexpensive substance without a need for a special technique and which among other things enables comprehensive profiling kinetics of various protein kinases. [Means for Solving Problems] A method for analysis of protein kinase activity by detecting phosphorylation of at least one peptide which serves as a substrate for at least one protein kinase and is immobilized on the metal thin film in a basal plate of an array, characterized in that , in detecting the phosphorylation, the phosphorylated peptide is treated with a polyamine-zinc complex represented by, e.g., formula (I) which has a molecular weight of 500 to 1,000 and is modified with biotin and then preferably with avidin or streptavidin.
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