Adenoviral vectors with two separate expression cassettes

摘要

The invention relates to recombinant replication-defective adenoviral vectors comprising two separate expression cassettes, each cassette enabling the high expression of a protein encoded by a nucleic acid of interest. Each cassette comprises a promoter and a polyadenylation signal. An important aspect of the recombinant adenoviruses according to the invention is that they remain stable upon numerous passages in cell culture. Assays to monitor the stability of such adenoviruses over multiple passages are provided.