| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for a polymerase chain reaction to use a template nucleic acid, at least one primer, deoxyribonucleoside triphosphates and a polymerase having proofreading activity. <P>SOLUTION: The invention provides a method for significantly increasing the efficiency of a DNA polymerase having proofreading activity and/or reducing or, in the optimal case, blocking the 3'-5'-exonuclease activity of the DNA polymerase. The target substrate used in the invention is in particular single stranded, linear oligonucleotides, hairpin oligonucleotides and RNA and DNA molecules. Furthermore, the invention discloses a kit containing a reagent necessary for the implementation of the polymerase chain reaction. <P>COPYRIGHT: (C)2007,JPO&INPIT |
