DEVICE AND METHOD FOR OBSERVING THE ORGANIZATION OF STUDIED CELLS CULTURED IN THE PRESENCE OF A CONCENTRATION GRADIENT OF CHEMOTACTIC MOLECULES

摘要

The device comprises a cell culture compartment (10) and a diffusion compartment (20) in fluid communication with the cell culture compartment (20) through a filtering element (22) for the progressive diffusion of chemotactic molecules contained in the diffusion compartment into a culture medium contained in the culture compartment, so as to maintain, in the culture compartment, a chemotactic molecules concentration gradient between a region (A) of the culture compartment (10) adjacent to the diffusion compartment (20) and a region (B) of the culture compartment (10) spaced from the diffusion compartment (20), wherein the ratio (R) of the volume of the culture compartment (10) usable for receiving a culture medium over the volume of the diffusion compartment (20) usable for receiving molecules is at least 15.