| 摘要 |
The invention relates especially to a method for the quantification of methylated DNA. According to said method, the DNA to be examined is first converted in such a manner that cytosine is converted to uracil while 5' methylcytosine remains unmodified. The converted DNA is then amplified in the presence of two real time probes. One probe is specific of the methylated or non-methylated state of the DNA and the other probe binds to the amplification product in a methylation-specific manner. The ratio of the signal intensities of the probes or the Ct values allow to calculate the methylation degree of the examined DNA. The inventive method is especially suitable for the diagnosis and prognosis of cancer and other diseases associated with a modification in the methylation status and for predicting the effects of drugs. |
