发明名称 |
Method for production of S-hydroxynitrile lyase by use of Escherichia coli |
摘要 |
A method for efficient production of an S-hydroxynitrile lyase by gene recombination using Escherichia coli is provided. A gene is formed by altering a codon in an S-hydroxyniitrylase gene originating in cassava (Manihot esculenta Crantz) without changing the amino acid sequence thereof till the frequency of codon usage wholly with an amino acid in Escherichia coli reaches a level of not less than 5%. According to the method for the production of an S-hydroxynitrile lyase by the use of the gene, the S-hydroxynitrile lyase can be produced in a large amount with an unusually high yield.
|
申请公布号 |
US7214523(B2) |
申请公布日期 |
2007.05.08 |
申请号 |
US20030738927 |
申请日期 |
2003.12.16 |
申请人 |
NIPPON SHOKUBAI CO., LTD. |
发明人 |
SEMBA HISASHI;ICHIGE EITA;MUKOUYAMA MASAHARU |
分类号 |
C12N15/09;C07H21/02;C12N1/20;C12N1/21;C12N9/88;C12N15/60 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|