| 摘要 |
The invention refers to biotechnology, in particular to a process for submerged cultivation of Penicillium expansum fungi strain and may be used in the microbiological industry for obtaining cellulosolytic preparations with an increased content of cellobiohydrolase. The process for submerged cultivation of Penicillium expansum CNMN FD 05 C fungi strain includes preparation of the spore suspension, treatment thereof during 30 min with low-intensity waves with ? 5,6 mm, emitted in periodic regime, inoculation of the treated suspension onto a nutrient medium with pH 4,5, with the following composition, mass %: KH2PO4 0,1, CaCl2 0,01, KCl 0,01, MgSO4À7H2O 0,03, NaNO3 0,25, FeCl3À6H2O 0,001, corn extract 1,5, corn stems 4,0, distilled water the rest and cultivation at the temperature of 28à30?C during 120 hours. The result of the invention consists in increasing the cellobiohydrolase biosynthesis.
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