| 摘要 |
<p>The present invention relates, in one aspect, to a nucleic acid probe for monitoring or detecting a target nucleic acid in a sample, wherein said probe: (i) comprises a reporter molecule and a quencher molecule capable of quenching the fluorescence of the reporter molecule; (ii) has a substantially closed structure in its unhybridised state such that the reporter is substantially quenched; (iii) is modified at the 3' terminus to prevent extension by a nucleic acid polymerase; and (iv) is or is capable of being substantially hydrolysed bythe nucleic acid polymerase, and wherein the nucleic acid probeJias: (i) a target annealing temperature of from about 64°C to about 75°C; and (ii) a melting temperature of from about 45°C to about 58°C.</p> |
